ExpOptimizer Codon Optimization Tool is used to optimize codons for recombinant gene expression in mainstream hosts. Its optimization parameters include more than ten key parameters in transcription and translation processes.

According to the algorithm, each calculation will randomly sample and return local optimal results, so the results returned from each submission will have differences.

Please enter the coding region (CDS) of the gene, the length must be a multiple of 3; if you input a protein sequence, please select the sequence type first, then input.

Sequence Type

Sequence Input

Expression Host

Restriction Enzyme Site Exclusion

Optimization Parameter Description

  • Codon Usage Preference
  • 5' Region Optimization (Translation Initiation Efficiency)
  • DNA Repeat Sequences
  • mRNA Secondary Structure
  • GC Content
  • SD Sequence
  • Exclude Specified Restriction Enzyme Sites